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User:University of Washington/18 July 2008
From 2008.igem.org
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Continued troubleshooting of PCR. Since all previous attempts to PCR have failed, attempted a control reaction using standard BioBrick primers on a part which has been both transformed and sequenced by our team. | Continued troubleshooting of PCR. Since all previous attempts to PCR have failed, attempted a control reaction using standard BioBrick primers on a part which has been both transformed and sequenced by our team. | ||
| - | Made glycerol stock of BW25141/pKD78. Mini-prepped pKD78. Now have Lambda Red recombinatory expression vector--some positive news. | + | Made glycerol stock of BW25141/pKD78. Mini-prepped pKD78 x2. Only the second mini-prep electrophoresed as expected (~6 kb), first mini-prep may be compromised. Now have Lambda Red recombinatory expression vector--some positive news. |
== AHL synthesis in yeast (Bryan) == | == AHL synthesis in yeast (Bryan) == | ||
Latest revision as of 23:10, 21 July 2008
Lambda Red Recombineering of RP4 (Bryan)
Continued troubleshooting of PCR. Since all previous attempts to PCR have failed, attempted a control reaction using standard BioBrick primers on a part which has been both transformed and sequenced by our team.
Made glycerol stock of BW25141/pKD78. Mini-prepped pKD78 x2. Only the second mini-prep electrophoresed as expected (~6 kb), first mini-prep may be compromised. Now have Lambda Red recombinatory expression vector--some positive news.
AHL synthesis in yeast (Bryan)
Attempted to rescue part C0061 from both 2007 & 2008 distribution by PCR amplification using standard BioBrick primers.
Klavins lab had success transforming part C0161. Obtained an ON broth culture.
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