EPF-Lausanne/24 July 2008

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Started do creat our simulation model using SimBiology. Have to find all the parameters values to get some useful results.
Started do creat our simulation model using SimBiology. Have to find all the parameters values to get some useful results.
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On the morning, we prepare cell to be electro competent(25 aliquots). We make same protocol as yesterday but we incube it only 2:20 .  
+
On the morning, we prepared cells to be electrocompetent(25 aliquots). We made the same protocol as yesterday but we incubated the cells only 2:20. The OD600 was already at 0.48.  
-
On afternoon, we do electro-transformation three time(1.25kV). We do with - PuC18, - 1020 2C and - 1003 7A.
+
On the afternoon, we did three electro-transformations at 1.25kV :  with - PuC18, - 1020 2C and - 1003 7A.
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We received swipes stocks of our parts. We let them to grow overnight in 1.5ml of LB + specific antibiotic.

Revision as of 12:04, 25 July 2008

Started do creat our simulation model using SimBiology. Have to find all the parameters values to get some useful results.

On the morning, we prepared cells to be electrocompetent(25 aliquots). We made the same protocol as yesterday but we incubated the cells only 2:20. The OD600 was already at 0.48.

On the afternoon, we did three electro-transformations at 1.25kV : with - PuC18, - 1020 2C and - 1003 7A.

We received swipes stocks of our parts. We let them to grow overnight in 1.5ml of LB + specific antibiotic.

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