Minnesota/27 July 2008

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(New page: {|style="align:left" width="965" |- |''' Back to Notebook Home''' |- |'''Go to Previous Day (July 26)'''|| width=158|'''[[...)
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|'''1. Spec the plasmid prep products:''' Place 10 samples into 10 separate plate wells. 11 wells total were used: 1st well was the control so it had 36uL of distilled water and 4uL of elution buffer (since want everything except DNA to be control group), then 2-11 wells had 1-10 plasmid prep products. 2-11 wells had 36uL of distilled water added and 4uL of plasmid prep products. Results:
|'''1. Spec the plasmid prep products:''' Place 10 samples into 10 separate plate wells. 11 wells total were used: 1st well was the control so it had 36uL of distilled water and 4uL of elution buffer (since want everything except DNA to be control group), then 2-11 wells had 1-10 plasmid prep products. 2-11 wells had 36uL of distilled water added and 4uL of plasmid prep products. Results:
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|a. (1) Lac I #1 = [DNA] ng/uL is 45, [DNA] ug/uL is 0.045    HIGH/GOOD
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|'''a.''' (1) Lac I #1 = [DNA] ng/uL is 45, [DNA] ug/uL is 0.045    HIGH/GOOD
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|b. (2) Lac I = [DNA] ng/uL is 35, [DNA] ug/uL is 0.035    HIGH/GOOD
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|'''b.''' (2) Lac I = [DNA] ng/uL is 35, [DNA] ug/uL is 0.035    HIGH/GOOD
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|c. (3) Lac I = [DNA] ng/uL is 35, [DNA] ug/uL is 0.035    HIGH/GOOD
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|'''c.''' (3) Lac I = [DNA] ng/uL is 35, [DNA] ug/uL is 0.035    HIGH/GOOD
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|d. (4) BV:Lac #2 = [DNA] ng/uL is 10, [DNA] ug/uL is 0.001  LOW/POOR
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|'''d.''' (4) BV:Lac #2 = [DNA] ng/uL is 10, [DNA] ug/uL is 0.001  LOW/POOR
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|e. (5) BV:Tet #1 = [DNA] ng/uL is 0, [DNA] ug/uL is 0.00      NONE/BAD
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|'''e.''' (5) BV:Tet #1 = [DNA] ng/uL is 0, [DNA] ug/uL is 0.00      NONE/BAD
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|f. (6) BV:Tet #2 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005    LOW/POOR
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|'''f.''' (6) BV:Tet #2 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005    LOW/POOR
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|g. (7) BV:Tet/p22 #1 =
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|'''g.''' (7) BV:Tet/p22 #1 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005    LOW/POOR
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|'''h.''' (8) BV:Tet/p22 #1 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005    LOW/POOR
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|'''i.''' (9) BV:Tet/p22 #2 = [DNA] ng/uL is 335, [DNA] ug/uL is 0.335    HIGH/GOOD
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|'''j.''' (10) BV:Tet/p22 #2 = [DNA] ng/uL is 15, [DNA] ug/uL is 0.015    LOW/POOR
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|'''2. Digest Rxn:''' Using #'s: 1, 2, 6, 9, and 10 from above. Also using: p22cII, LAMBDAcI, RFP from different plasmid prep product days. Follow the table below:

Revision as of 22:08, 27 July 2008

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1. Spec the plasmid prep products: Place 10 samples into 10 separate plate wells. 11 wells total were used: 1st well was the control so it had 36uL of distilled water and 4uL of elution buffer (since want everything except DNA to be control group), then 2-11 wells had 1-10 plasmid prep products. 2-11 wells had 36uL of distilled water added and 4uL of plasmid prep products. Results:
a. (1) Lac I #1 = [DNA] ng/uL is 45, [DNA] ug/uL is 0.045 HIGH/GOOD
b. (2) Lac I = [DNA] ng/uL is 35, [DNA] ug/uL is 0.035 HIGH/GOOD
c. (3) Lac I = [DNA] ng/uL is 35, [DNA] ug/uL is 0.035 HIGH/GOOD
d. (4) BV:Lac #2 = [DNA] ng/uL is 10, [DNA] ug/uL is 0.001 LOW/POOR
e. (5) BV:Tet #1 = [DNA] ng/uL is 0, [DNA] ug/uL is 0.00 NONE/BAD
f. (6) BV:Tet #2 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005 LOW/POOR
g. (7) BV:Tet/p22 #1 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005 LOW/POOR
h. (8) BV:Tet/p22 #1 = [DNA] ng/uL is 5, [DNA] ug/uL is 0.005 LOW/POOR
i. (9) BV:Tet/p22 #2 = [DNA] ng/uL is 335, [DNA] ug/uL is 0.335 HIGH/GOOD
j. (10) BV:Tet/p22 #2 = [DNA] ng/uL is 15, [DNA] ug/uL is 0.015 LOW/POOR
2. Digest Rxn: Using #'s: 1, 2, 6, 9, and 10 from above. Also using: p22cII, LAMBDAcI, RFP from different plasmid prep product days. Follow the table below:
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