Minnesota/1 August 2008

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1. Ran the terminator digest out on a gel: After which we were unable to see a band the appropriate length, so we simply cut out the gel where we expected the band to be (terminator is very small - so hard to see). Gel purified - then used this in our next ligation. When spec'ed the purified gel terminator product, there were 2.5ngram/uL of DNA concentration.

2. Currently have two parts that are base vector-promoter-gene, and one that is base vector-promoter: We are currently ligating the terminator to the two parts that have vector-promoter-gene.

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+|'''1. Ran the terminator digest out on a gel:''' After which we were unable to see a band the appropriate length, so we simply cut out the gel where we expected the band to be (terminator is very small - so hard to see). Gel purified - then used this in our next ligation. When spec'ed the purified gel terminator product, there were 2.5ngram/uL of DNA concentration.
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+|'''2.''' Currently have two parts that are base vector-promoter-gene, and one that is base vector-promoter: We are currently ligating the terminator to the two parts that have vector-promoter-gene.