Minnesota/4 August 2008

From 2008.igem.org

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|'''1. Send in products _______ for sequencing'''
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|'''1. Figure out what going to send in products for sequencing'''
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|'''2. Meeting with Yiannis:''' (1) Make sure powerpoint does not exceed 30 minutes, send it to Yiannis on Wednesday for him to review. (2) Write paper with materials and methods that can point to specific papers that we followed instead of writing out entire procedures. (3) Have iGEM requirements all finished before week ends.
|'''2. Meeting with Yiannis:''' (1) Make sure powerpoint does not exceed 30 minutes, send it to Yiannis on Wednesday for him to review. (2) Write paper with materials and methods that can point to specific papers that we followed instead of writing out entire procedures. (3) Have iGEM requirements all finished before week ends.

Revision as of 18:36, 5 August 2008

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1. Figure out what going to send in products for sequencing
2. Meeting with Yiannis: (1) Make sure powerpoint does not exceed 30 minutes, send it to Yiannis on Wednesday for him to review. (2) Write paper with materials and methods that can point to specific papers that we followed instead of writing out entire procedures. (3) Have iGEM requirements all finished before week ends.
3. Ligations: Ligate the digested products from 08-01-08. Follow the table below:


Parts 10x Buffer H20 prev. lig. + BV DNA Insert DNA T4 DNA Ligase Total
BV + Tet/p22 + RFP + Term 4.0uL 4.0uL 5.0uL 23.0uL 4.0uL 40.0uL
BV + Tet pro + LAMBDAcI + Term 4.0uL 4.0uL 5.0uL 23.0uL 4.0uL 40.0uL
BV + Lac/LAMBDA + GFP 1 4.0uL 4.0uL 5.0uL 23.0uL 4.0uL 40.0uL
BV + Lac/LAMBDA + GFP 2 4.0uL 4.0uL 5.0uL 23.0uL 4.0uL 40.0uL