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TUDelft/31 July 2008
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Cells put into culture yesterday were used to inoculate 200ml of LB. This was grown to an OD of 0.4. This was used to make glycerol stocks (60% glycerol was used, the protocol is on OpenWetWare). Not all 200ml was used for this, the surplus was discarded. | Cells put into culture yesterday were used to inoculate 200ml of LB. This was grown to an OD of 0.4. This was used to make glycerol stocks (60% glycerol was used, the protocol is on OpenWetWare). Not all 200ml was used for this, the surplus was discarded. | ||
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Revision as of 10:18, 26 August 2008
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31th July 2008
Cells put into culture yesterday were used to inoculate 200ml of LB. This was grown to an OD of 0.4. This was used to make glycerol stocks (60% glycerol was used, the protocol is on OpenWetWare). Not all 200ml was used for this, the surplus was discarded.
