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Edinburgh/6 August 2008
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li> | ||
<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li> | ||
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li> | ||
| - | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li> | + | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li> |
| + | <li><a href="https://2008.igem.org/Team:Edinburgh/Results" title="Results" rel="dropmenu1_a"><span>Results</span></a></li> | ||
| + | <li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Parts" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li> | ||
| + | <li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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| - | === | + | <table> |
| - | ==== Wednesday 6 August 08 | + | <html> |
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| + | </table> | ||
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| + | {| align="left" | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
| + | |} | ||
| + | |||
| + | :::: '''[[Edinburgh/5_August_2008|< Previous Entry]]''' | ||
| + | |||
| + | == Week 8 == | ||
| + | === Wednesday 6 August 08 === | ||
* '''L31''' ligation of P36 (glgC-mut1,2, hopefully, could be P39) to Edinbrick1 completed. (OG) | * '''L31''' ligation of P36 (glgC-mut1,2, hopefully, could be P39) to Edinbrick1 completed. (OG) | ||
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* Overnight culture made from plate 93 (pSB1A2+rbs+''crtB'') ready for maxiprep tomorrow. (AM, AH) | * Overnight culture made from plate 93 (pSB1A2+rbs+''crtB'') ready for maxiprep tomorrow. (AM, AH) | ||
* Analytical digests using a) EcoRI, b) EcoRI/PstI of M70, M71 (both pSB1A2+''glgC''-mut1,2) and M92 (pSB1A2 | * Analytical digests using a) EcoRI, b) EcoRI/PstI of M70, M71 (both pSB1A2+''glgC''-mut1,2) and M92 (pSB1A2 | ||
| - | +''cex''). Run on '''gel 39'''. Results do not look good. Bands are wrong size to correspond to vector and no bands match an expected insert size. (CF) | + | +''cex''). Run on '''gel 39'''. Results do not look good. Bands are wrong size to correspond to vector and no bands match an expected insert size. (CF)<br /> |
| + | <br /> | ||
| + | |||
| + | :::: '''[[Edinburgh/7_August_2008|Next Entry >]]''' | ||
Revision as of 10:20, 28 August 2008
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Week 8
Wednesday 6 August 08
- L31 ligation of P36 (glgC-mut1,2, hopefully, could be P39) to Edinbrick1 completed. (OG)
- Primers have arrived for pCstA. Chris carried out the PCR (that would be P51). Unsuccessful. (CF)
- Digestion of M67 (pSB1A2+rbs+crtB) with EcoRI/SpeI and M50 (pSB1A2+rbs+crtI) with EcoRI/XbaI, in preparation for combining the two with the former as insert and the latter as vector. (CF)
- Not enough time to purify/ligate due to high demand for agarose gel following lunchtime. Shall do tomorrow morning at 9. (AM)
- Re-digestion of M97, M98, M99 and M101 (all pSB1A2+cenA) and M105 and M108 (pSB1A2+cex) with a) EcoRI, b) EcoRI/PstI, and M92 (pSB1A2+cex) with a) PstI, b) EcoRI/PstI. All run on gel 38 (because gel 37 did not look good). (Yan)
- Transformation of L30 (Self-ligation of BABEL2+glgC-mut1,2,3) (plates 89 and 90) and L31 (glgC-mut1,2 to Edinbrick1) (plates 91 and 92) onto B/W plates. (HX)
- Maxipreps X6 (as M50, pSB1A2+rbs+crtI) and X7 (as M63: pSB1A2-+rbs+crtE) made. (AM)
- Overnight culture made from plate 93 (pSB1A2+rbs+crtB) ready for maxiprep tomorrow. (AM, AH)
- Analytical digests using a) EcoRI, b) EcoRI/PstI of M70, M71 (both pSB1A2+glgC-mut1,2) and M92 (pSB1A2
+cex). Run on gel 39. Results do not look good. Bands are wrong size to correspond to vector and no bands match an expected insert size. (CF)
