Team:University of Alberta/Plant Project

From 2008.igem.org

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(IGEM's First Binary Vector)
(IGEM's First Binary Vector)
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Our Binary Vector is a modified pCambia 0380 binary vector [[http://www.cambia.org/daisy/cambia/2037/version/1/part/4/data/pCAMBIA0380.pdf?branch=main&language=default| link]]
Our Binary Vector is a modified pCambia 0380 binary vector [[http://www.cambia.org/daisy/cambia/2037/version/1/part/4/data/pCAMBIA0380.pdf?branch=main&language=default| link]]
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We have added in our own poly linker which includes the proper prefix, suffix and primers to coincide with the IGEM regulations. We also added a CAM resistance cassette.
==The First Plant Project Ever Submitted to IGEM==
==The First Plant Project Ever Submitted to IGEM==

Revision as of 23:01, 4 June 2008

IGEM's First Binary Vector

Binary Vector.png

Our Binary Vector is a modified pCambia 0380 binary vector link We have added in our own poly linker which includes the proper prefix, suffix and primers to coincide with the IGEM regulations. We also added a CAM resistance cassette.

The First Plant Project Ever Submitted to IGEM

We hope to demonstrate that using biobricks in plants is not only useful but feasible in the restraints of this competition. We also hope to use the tools that we make to insert our toxin detection vector into plants.

Plant Protocol

Growing Plants
Selection of Primary Transformants
Transformation Protocol