From 2008.igem.org
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| - | <p>1. Plasmid isolation from previous day's colonies.<br> | + | <p>Plasmid isolation from colonies.<br> |
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| - | 2. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI<br>
| + | Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.<br> |
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| - | 3. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI<br>
| + | Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.<br> |
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| - | 4. Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI<br>
| + | Control digestion of pMPM-T5 + T7 RNA-polymerase with EcoRI.<br> |
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| - | 5. Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI<br>
| + | Control digestion of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.<br> |
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| - | 6. DNA electrophoresis of digestion products<br>
| + | DNA gel electrophoresis of digestion products.<br> |
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| - | 7. Choice of proper colonies of transformants<br>
| + | Picking out proper colonies of transformants.<br> |
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Revision as of 13:48, 26 September 2008
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Plasmid isolation from colonies.
Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.
Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.
Control digestion of pMPM-T5 + T7 RNA-polymerase with EcoRI.
Control digestion of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.
DNA gel electrophoresis of digestion products.
Picking out proper colonies of transformants.
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