Team:Michigan/8 October 2008
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+ | <div align=left>[https://2008.igem.org/wiki/index.php?title=Team:Michigan/6_October_2008 <font size=3 color=navy><< Previous Entry >></font>]</div> <div align=right>[https://2008.igem.org/wiki/index.php?title=Team:Michigan/8_October_2008 <font size=3 color=navy><< Next Entry >></font>]</div> | ||
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+ | =October 8, 2008= | ||
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+ | ==<B> Three Weeks to Send Parts.... </B>== | ||
+ | *Tried transforming a few BioBricks that had GFP in them; didn't get many colonies at all... - grew up cultures of those colonies and re-transformed | ||
+ | *pTRTp midiprep didn't work; weird band lengths came out gel; proceeding with a new strategy for our activator module: | ||
+ | *We'll be using BBa R0010 (lac repressible promoter) and pCLOCK1 (nifHp in LeuLP) - we'll drop in NifA behind these promoters. We'll then transfer to a landing pad (in case of the lac promoter driving NifA) and then put this piece on the chromosome. | ||
+ | *Work done: | ||
+ | *Digested pCLOCK1 with NdeI/SpeI, NifA with NdeI/SpeI, another NifA sample with Xba/Spe, and BBa0010 with Spe. | ||
+ | *Transformed BioBricks in XL-1 blue competent cells | ||
+ | *Picked/Grew up Cultures of colonies we got off our plates | ||
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+ | {| align="center" | ||
+ | |-valign="top" | ||
+ | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=06}} | ||
+ | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=07}} | ||
+ | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=08}} | ||
+ | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=09}} | ||
+ | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=10}} | ||
+ | |align="center" width="150pt"|{{#calendar: title=Team:Michigan |year=2008 | month=11}} | ||
+ | |} | ||
+ | |} |
Revision as of 22:19, 8 October 2008
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October 8, 2008Three Weeks to Send Parts....
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