Team:Warsaw/Calendar-Main/6 September 2008

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<p><ol><li>Results of mutageneses:
<p><ol><li>Results of mutageneses:
<ul>
<ul>
-
<li>deletion mutagenesis:</li>
+
<li>ADelL+KpnI and ADelP primers mutagenesis:</li>
<ul>
<ul>
-
<li>pACYClac+ompA-A-omega: 5 colonies</li>
+
<li>pACYClac+ompA-A-omega: <b>5</b> colonies</li>
-
<li>pACYClac+ompa-A-alpha: 10 colonies</li>
+
<li>pACYClac+ompa-A-alpha: <b>10</b> colonies</li>
-
<li>pACYClac+ ompa-alpha-A: 3 colonies</li>
+
<li>pACYClac+ ompa-alpha-A: <b>3</b> colonies</li>
</ul>
</ul>
-
<li>mutagenesis:</li>
+
<li>AMutL+KpnI and AMutP mutagenesis:</li>
<ul>
<ul>
-
<li>pACYClac+ompA-A-omega: 20 colonies</li>
+
<li>pACYClac+ompA-A-omega: <b>20</b> colonies</li>
-
<li>pACYClac+ompa-A-alpha: 12 colonies</li>
+
<li>pACYClac+ompa-A-alpha: <b>12</b> colonies</li>
-
<li>pACYClac+ ompa-alpha-A: 17 colonies</li>
+
<li>pACYClac+ ompa-alpha-A: <b>17</b> colonies</li>
</ul>
</ul>
</ul>  
</ul>  

Revision as of 13:20, 9 October 2008

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Protein A mutagenesis
Paweł

  1. Results of mutageneses:
    • ADelL+KpnI and ADelP primers mutagenesis:
      • pACYClac+ompA-A-omega: 5 colonies
      • pACYClac+ompa-A-alpha: 10 colonies
      • pACYClac+ ompa-alpha-A: 3 colonies
    • AMutL+KpnI and AMutP mutagenesis:
      • pACYClac+ompA-A-omega: 20 colonies
      • pACYClac+ompa-A-alpha: 12 colonies
      • pACYClac+ ompa-alpha-A: 17 colonies
  2. Each colony cultured overnight in LB+amp for further analyses




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