From 2008.igem.org
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| - | <h3>Protein A mutagenesis<br>Paweł</h3>
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| - | <p><ol><li>Isolation of plasmid DNA from overnight cultures (it was really tough work...)</li>
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| - | <li>Control digestion of isolated plasmids with KpnI and BamHI (if mutagenesis really occured, xxxbp band should be visible)</li>
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| - | <li>Results of digestion:</li>
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| - | <ul>
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| - | <li>ADelL+KpnI and ADelP primers mutagenesis:</li>
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| - | <ul>
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| - | <li>pACYClac+ompA-A-omega: <b>0</b> positive, <b>5</b> negative</li>
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| - | <li>pACYClac+ompa-A-alpha: <b>3</b> positive, <b>7</b> negative</li>
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| - | <li>pACYClac+ ompa-alpha-A: <b>1</b> positive, <b>2</b> negative</li>
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| - | </ul>
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| - | <li>AMutL+KpnI and AMutP mutagenesis:</li>
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| - | <ul>
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| - | <li>pACYClac+ompA-A-omega: <b>4</b> positive, <b>16</b> negative</li>
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| - | <li>pACYClac+ompa-A-alpha: <b>4</b> positive, <b>8</b> negative</li>
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| - | <li>pACYClac+ ompa-alpha-A: <b>2</b> positive, <b>15</b> negative</li>
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| - | </ul>
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| - | </ul>
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| - | <li>Plasmids which gave positive digestion results prepared for sequencing (one sample from each variant which gave positive result)</li>
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| - | </ol></p>
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Revision as of 15:28, 9 October 2008
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