Template:Team:UC Berkeley/Notebook/DV construction
From 2008.igem.org
(Difference between revisions)
Dirkvandepol (Talk | contribs) |
Dirkvandepol (Talk | contribs) (→K112704) |
||
Line 53: | Line 53: | ||
==K112704== | ==K112704== | ||
<pre> | <pre> | ||
- | + | Wobble PCR of dv011/dv012 (no template) (60 bp, EcoRI/BamHI/DpnI) | |
Sub into pBca1256 (EcoRI/BamHI) | Sub into pBca1256 (EcoRI/BamHI) | ||
Product is pBca1256-K112704 | Product is pBca1256-K112704 | ||
Line 62: | Line 62: | ||
GGATCCttaCGCTGTCCATGTGCTGGCGTTCGAATTTAGC | GGATCCttaCGCTGTCCATGTGCTGGCGTTCGAATTTAGC | ||
</pre> | </pre> | ||
+ | |||
==K112705== | ==K112705== | ||
<pre> | <pre> | ||
Mix dv013/dv014 (no template) (47 bp, | Mix dv013/dv014 (no template) (47 bp, |
Revision as of 23:37, 10 June 2008
Contents |
K112701
Construction of [<Phns>] Basic Part K112701 PCR dv001/dv004 on Phns (150 bp, gp = A) PCR dv003/dv006 on Phns (225 bp, gp = B) PCR dv005/dv002 on Phns (342 bp, gp = C) --------------------------------------------------- PCR mv001/dv006 on A+B (355 bp, gp = D) --------------------------------------------------- PCR dv001/dv002 on C+D (697bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+697) Product is pBca1256-K112701 ----------------------------------------------- dv001 Forward Biobricking of [<Phns>] CCATAgaattcATGagatctGAAATATAGCTGTGCCATCAGCC dv002 Reverse Biobricking of [<Phns>] CCATAggatccGCACGAAGAGTACGGATG dv003 Removing the 1st EcoRI site in [<Phns>] (F) GCCAGGAATGTAAGGgATTCAAAATTGTTC dv004 Removing the 1st EcoRI site in [<Phns>] (R) GAACAATTTTGAATcCCTTACATTCCTGGC dv005 Removing the 2nd EcoRI site in [<Phns>] (F) gctaaagaGttcgccgaagtagc dv006 Removing the 2nd EcoRI site in [<Phns>] (R) gctacttcggcgaaCtctttagc
K112705
==K112702== <pre> Construction of <His-Tag! Basic Part K112702 Mixing of dv007/dv008 (27bp, EcoRI/BamHI/DpnI) Sub into (vector?)pBca1256 (EcoRI/BamHI, 2472+27, L) Product is pBca1256-K112702 ----------------------------------------------- dv007 Forward biobricking of <His-Tag! CATCATCATCATCATCATtaaGGATCC dv008 Reverse biobriquing of <His-Tag! GGATCCttaATGATGATGATGATGATG
K112703
Mix dv009/dv010 on pBca9145-Bca1117 (41bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+41, L) Product is pBca1256-K112703 ----------------------------------------------- dv009 Forward Biobricking of His-Tag> ccataGAATTCatgAGATCTatgCATCATCATCATCATCAT dv010 Reverse Biobricking of His-Tag> ATGATGATGATGATGATGcatAGATCTcatGAATTCtatgg
K112704
Wobble PCR of dv011/dv012 (no template) (60 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112704 ---------------------------------------- dv011 Forward Biobricking of <S-Tag! GATCTAAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCA dv012 Reverse Biobricking of <S-Tag! GGATCCttaCGCTGTCCATGTGCTGGCGTTCGAATTTAGC
K112705
Mix dv013/dv014 (no template) (47 bp,