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Team:Warsaw/Calendar-Main/27 June 2008
From 2008.igem.org
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<p><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Clean-up</a> of OmpA_alpha and OmpA_omega digest products. </p> | <p><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Clean-up</a> of OmpA_alpha and OmpA_omega digest products. </p> | ||
| - | <h3>Cloning PCL products on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a>vector</h3> | + | <h3>Cloning PCL products on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a> vector</h3> |
<h4>Michał L., Ewa, Marcin</h4> | <h4>Michał L., Ewa, Marcin</h4> | ||
<ol> | <ol> | ||
<li>Gel electophoresis of PCL products (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/27_June_2008#fig1">Fig. 1</a>).</li> | <li>Gel electophoresis of PCL products (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/27_June_2008#fig1">Fig. 1</a>).</li> | ||
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Gel-out</a> of proper bands (alpha-A: 1500 bp and omega-A: 1440 bp).</li> | <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Gel-out</a> of proper bands (alpha-A: 1500 bp and omega-A: 1440 bp).</li> | ||
| - | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Restriction digest</a> of pKS vector and the PCL products with NotI and SacI (BamHI buffer) for 2 hours.</li> | + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Restriction digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a> vector and the PCL products with NotI and SacI (BamHI buffer) for 2 hours.</li> |
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> 1 hour.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> 1 hour.</li> | ||
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of Top10 strain and screening on Amp<sub>100</sub> plates.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of Top10 strain and screening on Amp<sub>100</sub> plates.</li> | ||
Revision as of 21:53, 12 October 2008
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Preparation of constructs with OmpA protein fusionsMichał K.Clean-up of OmpA_alpha and OmpA_omega digest products. Cloning PCL products on pKS vectorMichał L., Ewa, Marcin
Fig. 1.PCL product - Omega-A fusion.
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