Team:Warsaw/Calendar-Main/26 June 2008

From 2008.igem.org

(Difference between revisions)
Line 3: Line 3:
<html>
<html>
 +
 +
<html>
 +
 +
<h3>Change of the reporter from pZC with B-galactosidaze to GFP or RFP</h3>
 +
<h4>Piotr, Weronika</h4>
 +
<ol>
 +
<li>Isolation of pZC</li>
 +
 +
<li>Inoculation of E.coli TOP10 carrying nazwa (Gfp genetrator), nazwa (RFP generator)
 +
 +
</li></ol>
 +
 +
<h3>Preparation of constructs with OmpA protein fusions </h3><h4>Michał K.</h4>
<h3>Preparation of constructs with OmpA protein fusions </h3><h4>Michał K.</h4>

Revision as of 08:44, 16 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Change of the reporter from pZC with B-galactosidaze to GFP or RFP

Piotr, Weronika

  1. Isolation of pZC
  2. Inoculation of E.coli TOP10 carrying nazwa (Gfp genetrator), nazwa (RFP generator)

Preparation of constructs with OmpA protein fusions

Michał K.

Repetition of PCRs, gel-out isolations and digests from 17 June.

Polymerase Chain Ligation (PCL)

Michał L., Ewa, Marcin

ProductTemplatesPrimersProduct length
Alpha-A fusionAlpha-linker + linker-AAlphaL+SacI + AP+NotI 1500 bp
Omega-A fusionOmega-linker + linker-AOmegaL+SacI + AP+NotI 1440 bp


PCL program for fusion A with alpha and omega
TemperatureTime
94°C4:00
94°C0:3028 cycles
48°C-62°C0:45
68°C2:30
68°C10:00
4°Cinfinite