Team:Warsaw/Calendar-Main/11 June 2008

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<h3> Blue/white and rifampicin test </h3><h4>Michał L., Ewa, Piotr</h4>
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<h3> Blue/white and rifampicin test </h3><h4>Michał L., Ewa</h4>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> with:
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> with:
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<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a>.</li></ul>
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a>.</li></ul>
<li>Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</li>
<li>Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</li>
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<li>Plating transformants on liquid LB + Tetracycline + Ampicillin 30 μg/mL + 0.1% Ara</li>
 
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<h3> Rifampicin test </h3><h4> Piotr</h4>
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Plating transformants on liquid LB + Tetracycline + Ampicillin 30 μg/mL + 0.1% Ara
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Revision as of 13:39, 19 October 2008

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Blue/white and rifampicin test

Michał L., Ewa

  1. Transformation of E. coli TOP10 carrying pZC320 with:
  2. Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).
  3. Rifampicin test

    Piotr

    Plating transformants on liquid LB + Tetracycline + Ampicillin 30 μg/mL + 0.1% Ara