Team:Warsaw/Calendar-Main/19 June 2008

From 2008.igem.org

(Difference between revisions)
Line 8: Line 8:
<p> Inoculation of separate transformant colonies from plates with ligations: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> (liquid LB + kanamycin).</p>
<p> Inoculation of separate transformant colonies from plates with ligations: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> (liquid LB + kanamycin).</p>
-
<h3>The analysis of beta-galactosidase sequence from pZC</h3>
 
-
<h4>Piotr</h4>
 
There is no mutation in beta-galactosidase gene. Why the colonys are white???
There is no mutation in beta-galactosidase gene. Why the colonys are white???
-
<h3> Blue/white test </h3>
+
<li>The analysis of lacZ sequence from pZC<br>
-
<h4>Piotr</h4>
+
There is no mutation in beta-galactosidase gene. Why the colonys are white???</li>
-
<ol>
+
<li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br>
<li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br>

Revision as of 18:35, 19 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Preparation of constructs with OmpA protein fusions

Michał K.

Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).

There is no mutation in beta-galactosidase gene. Why the colonys are white???
  • The analysis of lacZ sequence from pZC
    There is no mutation in beta-galactosidase gene. Why the colonys are white???
  • Colony PCR.
    Template: DNA isolated from white colonies
    Primers: pZCseqL and pZCseqR

    Colony PCR program
    TemperatureTimeNo. of cycles
    94°C4:00
    94°C0:3028 cycles
    48°C0:45
    72°C1:30
    72°C10:00
    4°Cinfinite