Team:Warsaw/Calendar-Main/28 July 2008

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<h3>Cloning omega-A fusion on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a> (second attempt)</h3>
<h3>Cloning omega-A fusion on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a> (second attempt)</h3>
<h4>Michał L., Ewa, Marcin</h4>
<h4>Michał L., Ewa, Marcin</h4>
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<p>Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be trunctated and contain only one B domains. We inoculated bacteria containing this plasmid to obtain it for futher cloning. </p>
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<p>Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be trunctated and contain only one B-domain. We inoculated bacteria containing this plasmid to obtain it for futher cloning. </p>

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Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin

Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be trunctated and contain only one B-domain. We inoculated bacteria containing this plasmid to obtain it for futher cloning.