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Team:Chiba/Calendar-Home/22 August 2008
From 2008.igem.org
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===Team:Input=== | ===Team:Input=== | ||
'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''': | '''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''': | ||
| - | Minipreped the cultures(- | + | Minipreped the cultures(<--21/8). |
*[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007) | *[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007) | ||
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insert check:'''[[Team:Chiba/protocol/digestion|Digestion]]''' | insert check:'''[[Team:Chiba/protocol/digestion|Digestion]]''' | ||
| - | + | #[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006) | |
| - | + | #[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007) | |
| - | + | #[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007) | |
| - | + | #[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007) | |
| - | + | #[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006) | |
| - | + | #[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007) | |
[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)と[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)はsingleのみ、ほかはsingleとdoubleの両方。 | [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)と[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)はsingleのみ、ほかはsingleとdoubleの両方。 | ||
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<BR>混ぜ表 | <BR>混ぜ表 | ||
<table width="200" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="200" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
| - | <td width="257"></td> | + | <td width="257" >Sample No.</td> |
| - | <td> | + | <td>1-4</td><td>1-6</td> |
<tr> | <tr> | ||
| - | <td>dH<sub>2</sub>O</td> | + | <td>dH<sub>2</sub>O</td><td>14</td><td>25</td> |
| - | <td>14</td><td>25</td> | + | |
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 09:24, 24 October 2008
21 August 2008 <|> 23 August 2008
Contents |
Laboratory work
Team:Input
Mini prep: Minipreped the cultures(<--21/8).
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
- [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
- [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)
insert check:Digestion
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
- [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
- [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)と[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)はsingleのみ、ほかはsingleとdoubleの両方。
混ぜ表
| Sample No. | 1-4 | 1-6 |
| dH2O | 14 | 25 |
| 10×BSA | 7 | 10 |
| 10×NE | 7 | 10 |
| EcoRI | 3.5 | 5 |
| PstI | 3.5 | - |
UV照射実験(液体編)(~24)
-Ptrc-LuxR-Plux-cI-colE1-Amp-,-PcI-GFP-p15a-Cm-(BW)の2plasmidを使用。
グリストをつついて2ml培養×2(LB-Amp,Cm)(LB-Amp,cm,AHL100nM)
37℃で、12h培養後、濁ったらそのまま小さなプレートにLB培地ごと移してUVを照射する。UVの波長は254nm,UVランプまでの距離は8cm。
UVを照射して、15min,4h,8h,12h,たったら、両方のプレートからLB培地ごと20μlずつ採取しそれぞれ105希釈し、そこから20μlをそれぞれ(LB-Amp,Cm)(LB-Amp,cm,AHL100nM)のプレートに撒いた。
37℃で12h培養した後のコロニー数
| AHLなし | AHL100nM | |
| 15min | 136 | 0? |
| 4h | 40 | 31 |
| 8h | 10 | 0 |
| 12h | 0 | 0 |
いづれのプレートもAHLが入っていないものはGFPが確認できたが、AHL100nMのものは確認できなかった。
Team:Communication
- Transformation
- competent cells : XL10G
- BBa_J13002(2007) [http://partsregistry.org/Part:BBa_J13002]
- BBa_J13002(2006) [http://partsregistry.org/Part:BBa_J13002]
- BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
- BBa_J04500(2006) [http://partsregistry.org/Part:BBa_J04500]
- (Locate & 2 Punch ~iyama ver.~)
- BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
- BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
- BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
- BBa_J04500(2008) [http://partsregistry.org/Part:BBa_J04500] -> No colonies on plates
--->(24/8)Mini prep
Team:Output
- [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](Luciferase)
- [http://partsregistry.org/Part:BBa_F2620 BBa_F2620]
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]
- [http://partsregistry.org/Part:BBa_J33202 BBa_J33202](LacZ)
- PUC19
- PGEX VENUS YFP(GST fusion)
- PGEX VENUS YFP(GST fusion)
