Team:Warsaw/Calendar-Main/28 July 2008

From 2008.igem.org

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<h3>Cloning omega-A fusion on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a> (second attempt)</h3>
<h3>Cloning omega-A fusion on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a> (second attempt)</h3>
<h4>Michał L., Ewa, Marcin</h4>
<h4>Michał L., Ewa, Marcin</h4>
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<p>Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be truncated and contain only one B-domain. We inoculated bacteria containing this plasmid to obtain it for futher cloning. </p>
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<p>Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be truncated and contain only one B-domain (which we called 'delta A'). We inoculated bacteria containing this <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2Bomega-deltaA>plasmid</a> to obtain it for futher cloning. </p>
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<h4>Piotr</h4>
<h4>Piotr</h4>
<p>
<p>
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Single transformations of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with plasmids pET15b+Z-alpha and pET15b+Z-omega. Plating them on LB with chloramphenicol and ampicillin.</p></html>
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Single transformations of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with plasmids <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BZ-alpha>pET15b+Z-alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2Bhis%2BZ%2Bomega>pET15b+Z-omega</a>. Plating them on LB with chloramphenicol and ampicillin.</p></html>

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Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin

Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be truncated and contain only one B-domain (which we called 'delta A'). We inoculated bacteria containing this plasmid to obtain it for futher cloning.

Protein purification: Z-alpha i Z-omega

Piotr

Single transformations of Rosetta with plasmids pET15b+Z-alpha and pET15b+Z-omega. Plating them on LB with chloramphenicol and ampicillin.