Team:Warsaw/Calendar-Main/31 July 2008
From 2008.igem.org
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<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/2/2e/31_july.jpg" width=250/></a> <var><b>Fig. 1. </b>Control SacI/BamHI digests of isolated plasmids<br> | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/2/2e/31_july.jpg" width=250/></a> <var><b>Fig. 1. </b>Control SacI/BamHI digests of isolated plasmids<br> | ||
- | 1-4. digested plasmids pACYC177 + OmpA-omega- | + | 1-4. digested plasmids pACYC177 + OmpA-omega-ΔA-alpha<br> |
5. Marker<br></var> | 5. Marker<br></var> | ||
Latest revision as of 18:58, 26 October 2008
Cloning of truncated fragment of protein A (ΔA)Michał K.
Cloning of omega_ΔA DNA fragment to pACYC177+OmpA_alphaMichał K.
1-4. digested plasmids pACYC177 + OmpA-omega-ΔA-alpha 5. Marker Purification of proteins: Z-alpha and Z-omegaPiotr, Emilia
The arrow shows place of our overexpressed protein: 1. 22°C 0.5 mM IPTG 2. 22°C 0.1 mM IPTG 3. 22°C 1 mM IPTG 4. Marker 5. negative control 6. 37°C 0.1 mM IPTG 7. 37°C 0.5 mM IPTG 8. 37°C 1 mM IPTG Fig. 3. Pellets and supernatants from Z-alpha induction with various IPTG concentrations The arrow shows place of our overexpressed protein: 1. Marker 2. pellet negative control 3. supernatant negative control 4. pellet 37°C 0.1 mM IPTG 5. supernatant 37°C 0.1 mM IPTG 6. sonicate 22°C 0.1 mM IPTG 7. sonicate 22°C 0.5 mM IPTG 8. pellet 37°C 0.5 mM IPTG 9. supernatant 37°C 0.5 mM IPTG
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