Team:Warsaw/Calendar-Main/6 October 2008
From 2008.igem.org
(Difference between revisions)
Line 25: | Line 25: | ||
- | <h3>Preparation of pACYC177 + | + | <h3>Preparation of <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a></h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<ol> | <ol> | ||
<li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | <li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | ||
- | primers on colonies from plates with transformations <a href=http://partsregistry.org/ | + | primers on colonies from plates with transformations <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a> (annealing temperature - 55°C,90 s of elongation step). No visible bands for proper product.</li> |
- | <li>Inoculation of some colonies which grown on plate transformation: pACYC177 + | + | <li>Inoculation of some colonies which grown on plate transformation: <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a> to liquid LB + kanamycin. </li></ol> |
Revision as of 00:24, 27 October 2008
Preparation of pSB2K3 + linker_alpha (BBa_K103009)Michał K.
Preparation of pSB2K3 + linker_omega (BBa_K103013)Michał K.Inoculation of some colonies which grown on plate - pSB2K3 + linker_omega (BBa_K103013) to liquid LB + kanamycin. Preparation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016)Michał K.
Preparation of BioBricksMichał K.
|