Team:Warsaw/Calendar-Main/21 October 2008

From 2008.igem.org

(Difference between revisions)
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<h4>Michał K.</h4>
<h4>Michał K.</h4>
<ol>
<ol>
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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB2K3>pSB2K3</a> + pT7_alpha).</li>
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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB2K3>pSB2K3</a> + <a href=http://partsregistry.org/Part:BBa_K103019>alpha_linker under PT7 (BBa_K103019)</a>).</li>
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol>
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol>
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<ol>
<ol>
<li> Inoculation of colonies from plate with ligation of pACYC177 + OmpA_alpha_ to LB with kanamycin and RFP(?????) + AraC+pBAD+AID to LB with ampicillin. </li>
<li> Inoculation of colonies from plate with ligation of pACYC177 + OmpA_alpha_ to LB with kanamycin and RFP(?????) + AraC+pBAD+AID to LB with ampicillin. </li>
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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day RFP(?????) + AraC+pBAD+AID and 5kb(?????) + pT7_alpha.</li>
+
<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day RFP(?????) + AraC+pBAD+AID and 5kb(?????) + <a href=http://partsregistry.org/Part:BBa_K103019>alpha_linker under PT7 (BBa_K103019)</a>.</li>
-
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found for 5kb(?????) + pT7_alpha.</li>
+
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found for 5kb(?????) + <a href=http://partsregistry.org/Part:BBa_K103019>alpha_linker under PT7 (BBa_K103019)</a>.</li>
</ol>
</ol>

Revision as of 10:19, 27 October 2008

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Preparation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016)

Piotr

Inoculation of few more colonies from plate with ligation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016) (from 16 October) to LB with kanamycin.

Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day (pSB2K3 + alpha_linker under PT7 (BBa_K103019)).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Preparation of BioBricks

Michał K.

  1. Inoculation of colonies from plate with ligation of pACYC177 + OmpA_alpha_ to LB with kanamycin and RFP(?????) + AraC+pBAD+AID to LB with ampicillin.
  2. Isolation of plasmids from cultures inoculated on previous day RFP(?????) + AraC+pBAD+AID and 5kb(?????) + alpha_linker under PT7 (BBa_K103019).
  3. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found for 5kb(?????) + alpha_linker under PT7 (BBa_K103019).

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