Team:Warsaw/Calendar-Main/19 September 2008

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<h3>Preparation of <A href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103003>&Delta;A (BBa_K103003)</a></h3>
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<h3>Preparation of <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103003>&Delta;A (BBa_K103003)</a></h3>
<h4>Michał K., Piotr</h4>
<h4>Michał K., Piotr</h4>
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Revision as of 10:31, 27 October 2008

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MutD5 testing

Piotr

Testing MutD5 ampicillin resistance - MutD5 has not spontaneous ampicillin resistance mutations.

'Hunter and prey' system tests: Competition tests

Weronika

Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)

  2. Bacteria centrifuged and frozen

Preparation of ΔA (BBa_K103003)

Michał K., Piotr

  1. Gel electrophoresis to estimate concentration of purified DNA from previous day.
  2. Ligation of isolated DNA fragments of pSB1A3 and ΔA.
  3. E. coli TOP10 transformation with the above ligation.
  4. Plating on LB + ampicillin.

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