Team:Warsaw/Calendar-Main/26 September 2008

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<h3>Preparation of<a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103003>&Delta;A (BBa_K103003)</a></h3>
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<h3>Preparation of <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103003>&Delta;A (BBa_K103003)</a></h3>
<h4>Piotr</h4>
<h4>Piotr</h4>

Latest revision as of 10:47, 27 October 2008

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Mutagenesis of protein A

Paweł

  1. Results of mutageneses:
  2. Each colony cultured overnight in LB + kanamycin for further analyses.

Preparation of ΔA (BBa_K103003)

Piotr

  1. Isolation of plasmid from culture inoculated on previous day.
  2. Control digest of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

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