Team:PennState/diauxie/progress

From 2008.igem.org

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   <td>Promoter</td><td>pSB1A2</td><td>DH5&alpha;</td><td>W3110 &delta;xylB-G</td><td>W3110 &delta;xylB-R</td>
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Revision as of 19:23, 27 October 2008

Diauxie Elimination

Introduction
Implementation
Progress
Parts
References

Hormone Biosensors

Introduction
Smart Fold
Overview
Parts
References
Nuclear Fusion
Overview
Parts
References
Progress & Results

We have completed our first set of cloning phases and are conducting our first round of testing and modification. Each test construct (promoter + GFP) has been cloned into pSB1A2 and transformed into DH5α, W3110 ∆xylB-G, and W3110 ∆xylB-R. Preliminary induction studies were run to find the optimal induction time and to see in what range OD and fluorescence were linearly related.

PromoterpSB1A2DH5αW3110 ΔxylB-GW3110 ΔxylB-R
PN
P1
P3
P5

Test were then run to compare the level of induction between mixes of xylose, glucose and xylose, and glucose. The goal of this project is to obtain a noticeably higher level of induction when induced with xylose and glucose compared to the wildtype construct.