Team:Warsaw/Calendar-Main/6 October 2008
From 2008.igem.org
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103018>OmpA_linker_omega_linker under Plac (BBa_K103018)</a></h3> | <h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103018>OmpA_linker_omega_linker under Plac (BBa_K103018)</a></h3> | ||
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
- | <p> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + | + | <p> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + <a href=http://partsregistry.org/Part:BBa_K103018>OmpA_linker_omega_linker under Plac (BBa_K103018)</a> (without EcoRI site).</p> |
Revision as of 19:53, 27 October 2008
Preparation of linker_alpha (BBa_K103009)Michał K.
Preparation of linker_omega (BBa_K103013)Michał K.Inoculation of some colonies which grown on plate - pSB2K3 + linker_omega (BBa_K103013) to liquid LB + kanamycin. Preparation of OmpA-linker-omega-linker (BBa_K103016)Michał K.
Preparation of vector for pT7 constructsMichał K.Overnight selfligation of pET15b+OmpA_omega without XbaI. Preparation of OmpA_linker_omega_linker under Plac (BBa_K103018)Michał K.Overnight ligation of isolated DNA fragments pSB2K3 + OmpA_linker_omega_linker under Plac (BBa_K103018) (without EcoRI site). Preparation of AIDMichał K.
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