Team:Warsaw/Calendar-Main/6 October 2008
From 2008.igem.org
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- | <h3>Preparation of AID</h3> | + | <h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103001>AID(BBa_K103001)</a></h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
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<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#digest">Digest</a> of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> plasmid with XbaI and PstI (Tango buffer). <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">Dephosphorylation</a> (CIAP) of plasmid.</li> | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#digest">Digest</a> of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> plasmid with XbaI and PstI (Tango buffer). <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">Dephosphorylation</a> (CIAP) of plasmid.</li> | ||
<li> Gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band - 2200 bp.</li> | <li> Gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band - 2200 bp.</li> | ||
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</ol> | </ol> | ||
Revision as of 20:17, 27 October 2008
Preparation of linker_alpha (BBa_K103009)Michał K.
Preparation of linker_omega (BBa_K103013)Michał K.Inoculation of some colonies which grown on plate - pSB2K3 + linker_omega (BBa_K103013) to liquid LB + kanamycin. Preparation of OmpA-linker-omega-linker (BBa_K103016)Michał K.
Preparation of vector for pT7 constructsMichał K.Overnight selfligation of pET15b+OmpA_omega without XbaI. Preparation of OmpA_linker_omega_linker under Plac (BBa_K103018)Michał K.Overnight ligation of isolated DNA fragments pSB2K3 (from 1 October) + OmpA_linker_omega_linker under Plac (BBa_K103018) (without EcoRI site). Preparation of AID(BBa_K103001)Michał K.
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