Team:Warsaw/Calendar-Main/21 October 2008

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<h3>Preparation of OmpA_alpha_</h3>
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<h3>Preparation of OmpA-linker-alpha-linker</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
<p> Inoculation of colonies from plate with ligation (OmpA_alpha_) to LB with kanamycin. </p>
<p> Inoculation of colonies from plate with ligation (OmpA_alpha_) to LB with kanamycin. </p>

Revision as of 20:17, 27 October 2008

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Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

Inoculation of few more colonies from plate with ligation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016) (from 16 October) to LB with kanamycin.

Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day (pSB2K3 + alpha_linker under PT7 (BBa_K103019)).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Preparation of AraC+pBAD+AID

Michał K.

  1. Inoculation of few more colonies from plate with ligation of pSB1A3 + AraC+pBAD+AID to LB with ampicillin.
  2. Isolation of plasmids from cultures inoculated on previous day - pSB1A3 + AraC+pBAD+AID.
  3. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found.

Preparation of OmpA-linker-alpha-linker

Michał K.

Inoculation of colonies from plate with ligation (OmpA_alpha_) to LB with kanamycin.