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Revision as of 18:29, 29 October 2008

IPTG is an inducer which binds to LacI. LacI is a repressor that is constitutively expressed. It binds to

the promoter preventing the promoter from expressing an output, in

this case GFP.

In the absence of IPTG , LacI is constitutively expressed. Hence

the constitutive expression model from the previous

characterisation step can be assumed. At the same time, it can

bind to the promoter to repress it.

Repression.JPG

Equations plac.JPG

Evolution of LacI concentration

Conc LacI.jpg

The repressor only binds to the promoter when it is in its unbound

form.


Evolution of promoter concentration

Free promoters.jpg

Evolution of the promoter-LacI complex

Conc PLacI Complex.jpg

Evolution of GFP

Conc FP.jpg

dGFP[GFP] is the basal rate of GFP. This is caused by

the leakiness of the LacI-PLac complex when LacI does not repress

the promoter completely.