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Virginia/26 June 2008
From 2008.igem.org
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*redigest 6 parts for the three ligations | *redigest 6 parts for the three ligations | ||
*purify digestions | *purify digestions | ||
| - | *run gels to verify parts | + | *run NuSieve gels to verify parts |
*three runs of 3A assembly | *three runs of 3A assembly | ||
**RFP+GFP | **RFP+GFP | ||
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| - | [[Team:Virginia/Notebook]] | + | [[Team:Virginia/Notebook|Notebook]] |
Latest revision as of 15:35, 27 June 2008
Goals
- small broth of plated terminator
- large broth of E0240(RFP) and I715039(GFP)
- mini prepe PRE RFP and RET GFP for another ligation
- redigest 6 parts for the three ligations
- purify digestions
- run NuSieve gels to verify parts
- three runs of 3A assembly
- RFP+GFP
- Promoter+RBS
- BP1+terminator
Notes
- previous trials of 3A assembly failed
- digestion should have been fine so either ligation or purification steps went wrong
- iGEM agreed to send us CRUCIAL non functioning part from last year's library
- great success!!
