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Team:Chiba/Calendar-Home/18 August 2008
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Contents |
Laboratory work
Team:Input
バイオブリックパーツおこし
BBa_S01416
4E-iGEM2006 DNA plate 2
4E-iGEM 2007 Plate2
Transformation
z-competent Xl10G(Cm)
Plate-LB Kan
Team:communication
- PCR
- [http://partsregistry.org/Part:BBa_C0070 BBa_C0070(2008)]
- [http://partsregistry.org/Part:BBa_C0076 BBa_C0076(2008)]
- [http://partsregistry.org/Part:BBa_C0077 BBa_C0077(2008)]
- [http://partsregistry.org/Part:BBa_C0078 BBa_C0078(2008)]
- [http://partsregistry.org/Part:BBa_C0079 BBa_C0079(2008)]
- [http://partsregistry.org/Part:BBa_F2620 BBa_F2620(2007)]･･･control
| DNA Template | 1 |
| dNTP mix | 10 |
| Foward Primer | 5 |
| Reverse Primer | 5 |
| DNA polymerase | 1 |
| Thermopol Buffer | 10 |
| dH2O | 68 |
| TOTAL | 100μL |
- 95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
- --->Gel Check
|
|
competent cells : XL10G
- BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
- BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
- BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
- BBa_C0076(2006) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates(19/8)
- BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
- BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
- BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
- BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
- BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
- BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
- --->(20/8)Mini prep
Team:Output
no work
