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Template:Team:UC Berkeley/Notebook/DV construction
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Contents |
K112701
Construction of [<Phns>] Basic Part K112701 PCR dv001/dv004 on Phns (150 bp, gp = A) PCR dv003/dv006 on Phns (225 bp, gp = B) PCR dv005/dv002 on Phns (342 bp, gp = C) --------------------------------------------------- PCR mv001/dv006 on A+B (355 bp, gp = D) --------------------------------------------------- PCR dv001/dv002 on C+D (697bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+697) Product is pBca1256-K112701 ----------------------------------------------- dv001 Forward Biobricking of [<Phns>] CCATAgaattcATGagatctGAAATATAGCTGTGCCATCAGCC dv002 Reverse Biobricking of [<Phns>] CCATAggatccGCACGAAGAGTACGGATG dv003 Removing the 1st EcoRI site in [<Phns>] (F) GCCAGGAATGTAAGGgATTCAAAATTGTTC dv004 Removing the 1st EcoRI site in [<Phns>] (R) GAACAATTTTGAATcCCTTACATTCCTGGC dv005 Removing the 2nd EcoRI site in [<Phns>] (F) gctaaagaGttcgccgaagtagc dv006 Removing the 2nd EcoRI site in [<Phns>] (R) gctacttcggcgaaCtctttagc
K112702
Construction of <His-Tag! Basic Part K112702 Mixing of dv007/dv008 (27bp, EcoRI/BamHI/DpnI) Sub into (vector?)pBca1256 (EcoRI/BamHI, 2472+27, L) Product is pBca1256-K112702 ----------------------------------------------- dv007 Forward biobricking of <His-Tag! CATCATCATCATCATCATtaaGGATCC dv008 Reverse biobriquing of <His-Tag! GGATCCttaATGATGATGATGATGATG
K112703
Mix dv009/dv010 on pBca9145-Bca1117 (41bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+41, L) Product is pBca1256-K112703 ----------------------------------------------- dv009 Forward Biobricking of His-Tag> ccataGAATTCatgAGATCTatgCATCATCATCATCATCAT dv010 Reverse Biobricking of His-Tag> ATGATGATGATGATGATGcatAGATCTcatGAATTCtatgg
K112704
Mix dv011/dv012 on (template?) (60 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112704 ---------------------------------------- dv011 Forward Biobricking of <S-Tag! GATCTAAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCA dv012 Reverse Biobricking of <S-Tag! GGATCCttaCGCTGTCCATGTGCTGGCGTTCGAATTTAGC
