Preparation of constructs with OmpA protein fusions

Michał K.

Clean-up of OmpA_alpha and OmpA_omega digest products.

Cloning PCL products on pKS

Michał L., Ewa, Marcin:

1. Gel electophoresis of PCL products
2. Gel-out of proper bands
3. Restriction digest of pKS vector and the PCL products with NotI and SacI for 2 hours
4. Ligation 1 hour
5. Transformation of Top10 strain and screening on Amp₁₀₀ plates