Tests for ampicillin resistance given by protein added to medium

Piotr, Emila

1. Inoculation of all OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various cocnentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).

2. Inoculation of OmpA_A_alfa, Omp_A_omega, Omp_Z_omega, Omp_alfa and Omp_omega to LB + 0.25 mM IPTG and kanamycin.

3. Measurement of growth after 6 hours.