Team:NTU-Singapore/Modelling/Sensitivity Analysis

NTU@iGEM Project Introduction Methodology Team Introduction Team background Notebook Dry Lab Introduction ODEs used Modeling Constructs Parameters used Deterministic Modeling Sensitivity Analysis Stochastic Modeling Wetlab Introduction Materials and Equipment Protocols Verification of Detection & Lysis System Our New Parts Characterization Introduction Standard Promoters Characterization of pLacI-GFP pLacI-GFP images and clips Characterization of pLsrA-YFP New characterization Proposal Parameter Estimation & Correlations Acknowledgements

Lysis Sensitivity Analysis

The Sensitivity analysis revealed that the degradation rate of Proteins was a major factor and the previous assumption was not that accurate. The value for protein degradation was adjusted by assuming that the half time for protein degradation is 10 minutes. The new Lysis output according to the model is as follows.

As we can see, the output of protein has been drastically reduced from the previous modeling exercisefor the Lysis Protein Production system.