Alberta NINT/7 July 2008

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lab work (SD): 

            K102022.1 becomes K102022 and K102032.1 becomes K102032.
            Transformed XL1-B cells with K102022---------------LB + amp50 + IPTG + X-Gal + 20% glucose
                                         K102032---------------LB + amp50 + IPTG + X-Gal + 20% glucose
                                         K102032 + K102011-----LB + amp50 + kan35 + IPTG + X-Gal + 20% glucose
                                         K102032 + K102034-----LB + amp50 + kan35 + IPTG + X-Gal + 20% glucose
                                         K102032 + K102035-----LB + amp50 + kan35 + IPTG + X-Gal + 20% glucose
                                         K102032 + K102036-----LB + amp50 + kan35 + IPTG + X-Gal + 20% glucose
            Negative control plates are arabinose - and test plates are arabinose +

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