"
User:University of Washington/11 July 2008
From 2008.igem.org
LuxR from AraC and TetR
- Transformations of mutated AraC plasmid into XL1 Blue succeed!! Got many colonies in both of 2ul DNA plates, few in 15 ul DNA plates, none in negative control.
- Selected 6 colonies each from two 2-ul-DNA plates and inoculated onto two new plates (each divided into 6 regions) to get a single colony of each. (?) Left plates in the cabinet for two days growth.
Lambda Red Recombineering of RP4 (Bryan)
Repeated yesterday's PCR amplification of recombinant fragment. Included correct primer concentrations and reaction buffer this time. PCR failed anyway. Putative source of error: bad tetR template DNA.
Designed additional primers for future Lambda Red experiments.
AHL production in yeast (Bryan)
After yesterday's transformation of C0161 failed, making the third try using a new approach, concluded the part is faulty. Attempted transformation of related part C0061 LuxI-LVA into Top10.
Back to Team:University_of_Washington/Notebook#Notebook
