Team:Valencia/Notebook/July

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July
Mo Tu We Th Fr Sa Su
30  1  2  3  4  5  6
 7  8   9 10 11 12 13
14 15  16 17 18 19 20
21 22  23 24 25 26 27
28 29  30 31   1  2  3
August
Mo Tu We Th Fr Sa Su
28 29  30 31  1  2  3
 4  5   6  7  8  9 10
11 12  13 14 15 16 17
18 19  20 21 22 23 24
25 26  27 28 29 30 31
September
Mo Tu We Th Fr Sa Su
 1  2   3  4  5  6  7
 8  9  10 11 12 13 14
15 16  17 18 19 20 21
22 23  24 25 26 27 28
29 30  1 2 3 4 5
October
Mo Tu We Th Fr Sa Su
29 30   1  2  3  4  5
 6  7   8 9 10 11 12
13 14  15 16 17 18 19
20 21  22 23 24 25 26
27 28  29 30 31  1  2
November
Mo Tu We Th Fr Sa Su
27 28  29 30 31  1  2
 3  4   5  6  7  8  9
10 11  12 13 14 15 16
17 18  19 20 21 22 23
24 25  26 27 28 29 30



July 1st

- We prepared some glycerol stock from the four Saccharomyces cerevisiae strains we received from Eduardo Rial.

- We calibrated our calorimeters with hot water.

July 2nd

- We prepared our first SD medium.

- We calibrated our calorimeters with hot water.

July 4th

- We prepared some plates for inoculum.

July 7th

- We prepared some inoculum for our experiment the following day.

July 8th

- We carried out the pilot experiment.

July 9th

- We characterized culture growth in our calorimeters with just one strain.

July 10th

- Trying to see if there is basal rate of UCP1...

July 11th

- It looks like our Saccharomyces cerevisiae strains have a basal rate of UCP1... We'll have to repeat the experiment to be sure.

July 12th

- We obtained the same results.

July 14th

- The plate is completely dry,the water evaporeted...WHY???? the first unsolved mystery of valencia iGEM 2008.

July 15th

- We prepared inoculum.

July 16th

- Trying to find out which is the best moment for activating UCP1...

July 17th

- Our lab electricity went off during the night. How many experiments will have it ruined?

July 18th

- We prepared lots of plates with our SD medium...Unfortunately, we forgot to add glucose.

- We carried out the pilot experiment. We see weird oscillations.

July 20th

- We stopped the shaking and carried out the experiment again to see whether the oscillations were related to the shaking.

July 21st

- We carried out the pilot experiment again. Weird results, we need to repeat it.

July 22nd

- We repaired our calorimeters. They were the reason for yesterday weird results.

July 23rd

- We repeated the pilot experiment.

July 24th

- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste...

July 25th

- Another curious experiment was carried out: one thermocouple was covered with plasticine while the others weren't.

July 26th

- We obtain good results.

July 28th

- One of our calorimeters wasn't working. We repaired it and calibrated once again.

July 29th

- Our cultures haven't grown enough for the experiment. Temperature and medium was all right, we'll have to wait...