Team:Bologna/Protocols

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HOME THE PROJECT THE TEAM PARTS SUBMITTED TO THE REGISTRY MODELING NOTEBOOK


Contents

Plates preparation

  1. Autoclave LB medium with 2% agar.
  2. Cool at 50 °C to prevent agar polymerization.
  3. Before preparing plates add antibiotic (Ampicillin 1000x [ ] or Kanamicin 200x [ ]).
  4. Put about 20ml of medium per plate.
  5. Leave it solidify and store at 4°C.


Biobricks amplification

Transformation

Inoculation

Miniprep

Digestion reaction

Gel preparation

Electrophoretic run

Gel extraction


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