Team:ESBS-Strasbourg/4 August 2008
From 2008.igem.org
Contents |
DryLab
Modeling
WetLab
Katja
Construction of the operator BioBricks (GAL4O, LexAO, LacO, CIO, TetO)
- hybridisation of the ordered oligos
- ligation into the XbaI, PstI cutted vector pSB1A2 (using protocol 2c)
- transformation into competent TOP10 and plating on LB-Amp (using the heat-shock protocol)
Robin
- PCRs of Cin8, Tup1, Cln2, Hsl1, Cyc100-16