1. Separate transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin.

Paweł: Plasmid DNA isolation from overnight grown cultures. Restriction analysis (BamHI+NdeI) of 5 of them

Results: 2 of analysed plasmids contain OmpA+omega (~1350 bp band)

First of positive plasmids (now called pET15b+OmpA+omega) and Z (in GeneArt vector) digest with NdeI and NotI (overnight)