Purification of proteins: A-alpha, Z-alpha and Z-omega
Piotr

1. 100ml of medium inoculated with overnight culture, then used as an inoculum for 1l culture with presence of Ampicillin, Kanamycin and IPTG (according to protocol)

Protein A mutagenesis
Paweł

1. Results of mutagenesis: 0 colonies on each plate.
2. Mutagenesis repeated with modified conditions: fresh stock of dNTPs used and additional MgCl2 added.