Team:Warsaw/Calendar-Main/13 June 2008
From 2008.igem.org
Optimisation of primers from 10.06Michał K.
Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees. Gradient PCR products: 1, 14 and 27-DNA ladder; 2 to 8 - gradient PCR for Alpha carried out with 15 cycles; 9 to 16 - gradient PCR for Alpha carried out with 20 cycles; 17 to 23 - gradient PCR for Omega carried out with 15 cycles; 24 to 31 - gradient PCR for Omega carried out with 20 cycles; 32 to 38 - gradient PCR for OmpA carried out with 15 cycles; 39 to 46 - gradient PCR for OmpA carried out with 20 cycles. Product od each variant of PCR is in 7 lanes: 1st is the lower border annealing temperature (55 degrees), 6th is the higher annealing temperature (75 degrees) and 7th is negative control without template DNA.
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