"
Page
Discussion
View source
History
teams
Log in
Team:Warsaw/Calendar-Main/26 September 2008
From 2008.igem.org
Revision as of 23:31, 25 October 2008 by
Smaegol
(
Talk
|
contribs
)
(
diff
)
← Older revision
|
Latest revision
(
diff
) |
Newer revision →
(
diff
)
Attribution analyzer
/
Interactive list of topics
Mutagenesis of protein A
Paweł
Results of mutageneses:
ADelL+KpnI and ADelP primers mutagenesis:
pACYClac+OmpA-ΔA-omega
:
5
colonies
pACYClac+OmpA-ΔA-alpha
:
10
colonies
pACYClac+OmpA-omega-ΔA
:
3
colonies
AMutL+KpnI and AMutP mutagenesis:
pACYClac+OmpA-ΔA-omega
:
20
colonies
pACYClac+Ompa-ΔA-alpha
:
12
colonies
pACYClac+OmpA-omega-ΔA
:
17
colonies
Each colony cultured overnight in LB + kanamycin for further analyses.
Preparation of BioBricks
Piotr
Isolation
of plasmid from culture inoculated on previous day.
Control
digest
of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.