Team:PennState/Project

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This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your Team:Example namespace.



You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Example logo.png

Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)

Your team picture
Team Example 2


Home The Team The Project Parts Submitted to the Registry Modeling Notebook

(Or you can choose different headings. But you must have a team page, a project page, and a notebook page.)


Contents

Overall project

Our project aims to construct a bio sensor which will ultimately serve as a pre screening tool to detect the presence of phthalates in water sample. Recently phthalates have been shown to cause negative health effect in humans; some phthalates are even though to be carcinogenic. Analytical detection methods for these compounds are compound specific as well as very costly. Having a simple, and cheep tool to screen for phthalates as a general class of compounds would eliminate some of the cost and time involved in current detection methods.

We use the natural human nuclear hormone receptor protein that recognizes phthalates as an agonist (hPPARα), and expressing it heterologously in Escherichia Coli. Because of the complexity of this mammalian protein, expressing it in a prokaryote is difficult. We have two different strategies to express hPPARα and using it to detect phthalates in E. Coli.


Project Details

Part 2

The Experiments

Part 3

Results