Team:Edinburgh/Results/Edinbrick1

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Use of Edinbrick1 in preparation of BioBricks from PCR products

Edinbrick1 was developed by the Edinburgh 2006 iGEM team and was used extensively in both 2007 and 2008 to facilitate BioBrick construction. Although it was not developed by the 2008 team, we describe it here to increase awareness of it in case anyone else might find it useful.

Edinbrick1 consists of the standard BioBrick vector pSB1A2 with insert BBa_J33207 which includes a lac promoter and lacZ' minigene encoding the N-terminal 77 amino acids of the beta-galactosidase gene from E. coli B.