Team:Warsaw/Calendar-Main/19 September 2008

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MutD5 testing

Piotr

Testing MutD5 ampicillin resistance - MutD5 isn't resistant to ampicillin

'Hunter and prey' system tests: Competition tests

Emilia

  1. Samples of LB (0.25 mM IPTG; ampicillin 50 μl/ml) was inoculated with following strain combinations:

  2. Bacteria centrifuged and frozen

Preparation of ΔA (BBa_K103003)

Michał K., Piotr

  1. Gel electrophoresis to estimate concentration of purified DNA from previous day.
  2. Ligation of isolated DNA fragments of pSB1A3 and ΔA.
  3. E. coli TOP10 transformation with the above ligation.
  4. Plating on LB + ampicillin.