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21 May 2008
From 2008.igem.org
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(→Volunteers Scheduled for Today) |
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5-8 AS<br> | 5-8 AS<br> | ||
6-8 DL | 6-8 DL | ||
| + | Things to do: | ||
| + | Mini-preps of O/N's | ||
| + | Sequence gel purified fragments from yesterday | ||
| + | Transform I0500 | ||
| + | Digest J61003 with Xba/Pst to insert ORF's | ||
| + | Digest minipreps with Xba/Pst to insert into J61003 | ||
| + | Run gel of digests and excise correct fragments | ||
| + | Ligate vector/inserts | ||
| + | Transform ligation reactions | ||
| + | Lab clean up | ||
Revision as of 15:14, 21 May 2008
Volunteers Scheduled for Today
5-8 AS
6-8 DL
Things to do:
Mini-preps of O/N's
Sequence gel purified fragments from yesterday
Transform I0500
Digest J61003 with Xba/Pst to insert ORF's
Digest minipreps with Xba/Pst to insert into J61003
Run gel of digests and excise correct fragments
Ligate vector/inserts
Transform ligation reactions
Lab clean up
