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21 May 2008
From 2008.igem.org
(Difference between revisions)
Etphonejoe (Talk | contribs) (→Volunteers Scheduled for Today) |
Etphonejoe (Talk | contribs) (→Volunteers Scheduled for Today) |
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5-8 AS<br> | 5-8 AS<br> | ||
6-8 DL | 6-8 DL | ||
| - | Things to do: | + | Things to do:<br> |
| - | Mini-preps of O/N's | + | Mini-preps of O/N's<br> |
| - | Sequence gel purified fragments from yesterday | + | Sequence gel purified fragments from yesterday<br> |
| - | Transform I0500 | + | Transform I0500<br> |
| - | Digest J61003 with Xba/Pst to insert ORF's | + | Digest J61003 with Xba/Pst to insert ORF's<br> |
| - | Digest minipreps with Xba/Pst to insert into J61003 | + | Digest minipreps with Xba/Pst to insert into J61003<br> |
| - | Run gel of digests and excise correct fragments | + | Run gel of digests and excise correct fragments<br> |
| - | Ligate vector/inserts | + | Ligate vector/inserts<br> |
| - | Transform ligation reactions | + | Transform ligation reactions<br> |
| - | Lab clean up | + | Lab clean up<br> |
Revision as of 15:15, 21 May 2008
Volunteers Scheduled for Today
5-8 AS
6-8 DL
Things to do:
Mini-preps of O/N's
Sequence gel purified fragments from yesterday
Transform I0500
Digest J61003 with Xba/Pst to insert ORF's
Digest minipreps with Xba/Pst to insert into J61003
Run gel of digests and excise correct fragments
Ligate vector/inserts
Transform ligation reactions
Lab clean up
